DNA Extraction and Purification
Biochain Institute Inc Genomic DNA - Arrhythmia, infarct: Heart Atrium, right, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc cfPure MAX Cell-Free DNA Extraction Kit, 1 Kit (250 ml)/pk
BioChain's cfPure Cell Free DNA Extraction Kit is designed for rapid and efficient purification of circulating cell free DNA (cfDNA). The system utilizes silica-coated paramagnetic particles to purify cell free DNA from less than 1 mL to greater than 10 mL of serum or plasma. The buffers were developed to ensure efficient recovery of 100 bp - 500 bp DNA fragments in order to maximize recovery of cell free DNA. The recovered DNA is suitable for a wide range of down-stream applications, such as bisulfite sequencing, NGS, and qPCR.
Magbio Genomics Inc HighPrep Plasmid DNA Kit (96 preps)
Magbio's HighPrep™ Plasmid DNA kit is a magnetic beads based plasmid purification system for high throughput rapid isolation of high and low copy number plasmid DNA, BACs, PACs Cosmid and Fosmids. The system can be easily automated on many liquid handling workstation providing robustness and high quality plasmid DNA for subsequent downstream applications such as digestion and fluorescent DNA sequencing
Biochain Institute Inc SeqPure PCR Purification Kit (50 ml), 50 ml/pk
SeqPure PCR reagent is a post PCR and Next Gen library prep clean up system based on paramagnetic beads technology, designed for an efficient purification of PCR amplicons. The purification consists of removal of salts, primers, primer-dimers, dNTPs, as DNA fragments are selectively bound to the magnetic beads particles; and highly purified DNA is eluted with low salt elution buffer or water and can be used directly for downstream applications. The protocol can be adapted to your current liquid handling workstation (e.g. Beckman, Hamilton, Tecan, Caliper, Perkin Elmer, Agilent and Eppendorf) utilizing your current protocol as well as it can be performed manually.
Epoch Life Science Inc GenCatch™ Advanced PCR Cleanup Kit (50 preps) Uniquely formulated buffers, reinforced columns with collection tubes, along with protocols.
PCR purification kit. Silica membrane spin column based. Sample volume: 850 ul. Column binding capacity: 40 ug DNA. Minimum elution volume: 20ul. 18 month shelf life.
Qiagen, Inc. GENERATION DNA ELUTION
Generation DNA Elution Solution (500 ml)
Biotium Caldesmon, HMW (h-Caldesmon)(CALD1/820), CF740 conjugate, 0.1mg/mL
Caldesmon HMW is the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150 kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80 kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150 kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low
Biotium Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker), CF740 conjugate, 0.1mg/mL
Caldesmon HMW is the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120'150 kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70'80 kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This antibody recognizes only the 150 kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction. Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detectin
Magbio Genomics Inc HighPrep Plasmid DNA Kit (1920 preps)
Magbio's HighPrep™ Plasmid DNA kit is a magnetic beads based plasmid purification system for high throughput rapid isolation of high and low copy number plasmid DNA, BACs, PACs Cosmid and Fosmids. The system can be easily automated on many liquid handling workstation providing robustness and high quality plasmid DNA for subsequent downstream applications such as digestion and fluorescent DNA sequencing
Research Products International Corp Quick-DNA Miniprep Plus Kit, 200 Preps
The Quick-DNA Plus Kits are the easiest DNA isolation kits for high yield total DNA extraction (e.g., genomic, mitochondrial, viral) from cell culture, solid tissue, saliva, and any biological fluid sample. Innovative reagents and Zymo-Spin Technology allow for ultra-pure and concentrated genomic DNA (> 50 kb) to be eluted in as little as 10 µl. Zymo-Spin Columns ensure no buffer retention. Purified DNA is RNA-free,removing the need for RNase A treatments and ensuring accurate quantification for applications like library preparations. Isolated DNA is suitable for immediate use in sensitive downstream applications including qPCR, DNA-seq, arrays, and methylation analysis.
Quick & Easy: Simple 20 minute procedure.
Highest Yield: Recover 3x more DNA.
Ultra-Pure: Ready for qPCR, Next-Gen Sequencing, arrays, etc.
Epigentek Inc DNA Concentrator Kit (50 samples)
The DNA Concentrator Kit is a complete set of essential components which enables the experimenter to efficiently concentrate DNA from various DNA samples with low concentration of DNA including those from microdissection samples and paraffin-embedded tissues. The entire procedure can be completed within 2 minutes and is suitable for concentrating DNA from various DNA samples, especially from those with low concentration of DNA such as microdissection samples, paraffin-embedded tissues, restriction enzyme digestions, PCR reactions, and home-made minipreps.
Norgen Biotek Corp Bacterial Genomic DNA Isolation Kit
This kit provides a convenient and rapid method to isolate total DNA from fresh, frozen and preserved stool samples, including those preserved using Norgen’s Stool Nucleic Acid Collection and Preservation Tubes (Cat. 45660). The universal protocol conveniently allows for the isolation of total genomic DNA from all the various microorganisms and host cells found in the stool sample simultaneously. The kit removes all traces of humic acids and other inhibitors using a combination of chemical and physical homogenization and lysis. A simple and rapid spin column procedure is then used to further purify the DNA with high yields and molecular weights of up to 50 kb plus. The purified DNA is of the highest quality and is fully compatible with all downstream applications such as PCR, qPCR, Sequencing and microarrays since all humic acid substances and other PCR inhibitors are removed during the isolation process.