DNA Extraction and Purification
Epigentek Inc MethylFlash Methylated DNA 5-mC Quantification Kit (Colorimetric) (96 assays)
For absorbance-based quantitation of global DNA methylation in an ELISA-like format
Thomas Scientific DirectAmpT Genomic DNA Amplification Kits, Preparation Solution for DirectAmpT Tissue DNA Extraction & Amplification Kit, 100 reactions
Extract genomic DNA in 15 minutes Tissue, plant or seed Quick isolation and amplification for genotyping Highly specific PCR amplification Eliminates complicated purifications, centrifugation and extractions Denville DirectAmp™ Genomic DNA Amplification Kits contain all reagents for rapid extraction and amplification of genomic DNA from a variety of sources. These kits provide a simple and quick extraction method that eliminates protocol steps that call for liquid nitrogen freezing, enzyme digestions, hazardous organic extractions, column purification, centrifugation or precipitation. Each kit includes Denville Hot Start Blue Mastermix, noted for its highly specific amplification of targeted genomic DNA, without adding loading buffer or tracking dye for gel analysis. Although included as a standard kit component, Hot Start Blue Mastermix is also available separately.
Macherey-Nagel Resuspension Buffer RES‑EF, 1000 mL
Resuspension Buffer RES‑EF to be used with NucleoBond Xtra Midi / Maxi EF kits.
Zymo Research Corporation DNA/RNA Shield Lysis Tube w/ Swab (0.1 & 0.5 mm) (Microbe) 50 pack
DNA/RNA Shield Lysis and Collection tubes are designed for the streamlined collection, transport, and processing of biological specimen. They are pre-filled with DNA/RNA Shield and a lysis matrix tailored for tough-to-lyse- sample inputs from tissue to microbial samples. Collected samples do not require additional handling for processing and are ready for homogenization.
Zymo Research Corporation HostZERO Microbial DNA Kit (50 preps)
This kit uses a novel method to reduce the amount of contaminating host DNA by selectively lysing the eukaryotic cells and degrading this DNA prior to total DNA purification. Paired with Zymo Researchs non-biased purification technology, the HostZERO™ Microbial DNA Kit allows for the exclusive capture of DNA from living microbial cells in a biological sample.This new technology is able to reduce the presence of human DNA in a saliva sample from 65% (untreated sample) to less than 1% (treated sample, Figure 1). Concurrently, the depletion process utilizes the ZymoBIOMICS™ non-biased DNA isolation technology for accurate data representation (Figure 2). Finally, the HostZERO™ Microbial DNA Kit recovers the highest amount of bacterial DNA as compared to other methods (Figure 3). By removing the presence of host DNA and reducing bias in purification, the HostZERO™ Microbial DNA Kit produces the highest-quality data for microbial samples.
Boca Scientific Inc Xtreme DNA Purification Kit for Buccal Swabs and Saliva, 50 reactions
The Xtreme DNA Isolation Kit is a silica membrane-based spin column DNA purification kit designed to isolate genomic DNA from buccal swabs and saliva with minimal losses. The isolated DNA is highly purified with a high molecular weight and is free of trace PCR inhibitors. The DNA is suitable for use in even the most demanding downstream applications. This kit does not contain solvent-based chemicals.
Biotium DNA Gel Extraction Kit - 50 Columns
Biotium’s DNA Gel Extraction Kit is a silica-gel spin column based DNA extraction kit designed to purify DNA fragments from agarose gels. The kit has a maximum binding capacity of 10 ug DNA per column and can be used to purify DNA fragments from 40 bp to 40 kb. The kit is suitable for purifying DNA from agarose gels made with TAE or TBE buffer. The DNA Gel Extraction Kit removes GelRed and GelGreen Nucleic Acid Gel Stains, along with other commonly used nucleic acid gel stains. The purified DNA is compatible with common downstream applications such as transformation, sequencing, PCR, and restriction digests.
Norgen Biotek Corp Fungi/Yeast Genomic DNA Isolation Kit
Norgen's Fungi/Yeast Genomic DNA Isolation kit provides rapid spin column purification of genomic DNA from viable yeast cells, fungal spores or mycelium, and Gram-positive and Gram-negative bacteria. Genomic DNA is efficiently extracted from the cells by a combination of heat treatment, detergents and bead tubes (provided). An optional lyticase treatment allows for improved DNA yields with certain fungal and yeast species. Protocol time is typically less than 30 minutes. Recovered genomic DNA is of excellent yield and purity for any downstream application including PCR, qPCR, Restriction Fragment Length Polymorphism (RFLP), Amplified Fragment Length Polymorphism (AFLP), sequencing, SNP analysis and more.
Omega Biotek Inc E.Z.N.A Plasmid Mini Kit I, 200 preps, 1-5 mL bacterial culture, yield up to 30 μg DNA, elution volume 50 μL, HiBind Mini Column, V-spin, manual processing, safety feature: no phenol/chloroform
E.Z.N.A Plasmid Mini Kit I, 200 preps, 1-5 mL bacterial culture, yield up to 30 μg DNA, elution volume 50 μL, HiBind Mini Column, V-spin, manual processing, safety feature: no phenol/chloroform
Zymo Research Corporation Select-a-Size DNA MagBead Kit
This simple workflow allows for specific cutoffs thatcan be modified to suit reaction clean-up, left-sided, right-sided or even double-sided size selection. The Select-a-Size MagBead Buffer selectively binds fragments based on the volume added relative to the sample. Simply choose a desired cutoff to bind the target of interest onto the beads and remove species outside of this range. The desired DNA is easily eluted from the beads following a rapid wash regimen.
Beckman Coulter GENFIND V3 REAGENT KIT - 384 P
- High-throughput genomic DNA isolation kit
- Utilizes SPRI paramagnetic bead-based technology
- Efficient recovery of high-quality DNA
- Compatible with various sample types including whole blood
- Supports multiple anticoagulants including citrate and EDTA
- 384 preps for 200 µL samples, 192 preps for 400 µL samples
- High quality, intact genomic DNA output
- Applicable for PCR, genotyping, and Next-Generation Sequencing
- Manual and automated workflow options available
- Compatible with Beckman Coulter Biomek liquid handlers
- Liquid format for ease of use
- High throughput capability
- Research use only
- Minimizes hands-on time and pipetting errors
- High DNA recovery rate with reduced PCR inhibitors
Research Products International Corp ZR Plasmid Miniprep-Classic Kit, 100 Preps
The ZR Plasmid Miniprep-Classic is designed for efficient isolation of plasmid DNA from E. coli using a traditional 3-buffer (P1, P2, P3) procedure that is simple, rapid, and reliable. It features a modified alkaline lysis protocol together with Zymo-Spin technology to yield high quality plasmid DNA in minutes. The buffers are color-coded (red, green, yellow) for easy visualization of complete cell lysis and neutralization. The innovative Zymo-Spin llN columns yield transfection-grade plasmid DNA. Plasmid DNA purified using the ZR Plasmid Miniprep-Classic is well suited for use in restriction endonuclease digestion, sequencing, DNA ligation, cloning, PCR, bacterial transformation, transfection, etc.
High Purity: Eluted plasmid DNA is high quality, endotoxin-free and ready for all sensitive downstream applications including transfections and sequencing.
Colored Buffers: P1, P2, and P3 buffers are colored for easy visualization of complete lysis and neutralization.
Biotium Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker) (CALD1/1424R), CF488A conjugate, 0.1mg/mL
Caldesmon HMW is the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150 kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80 kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150 kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low