DNA Extraction and Purification
Norgen Biotek Corp FFPE RNA/DNA PURIFICATION KIT
Norgen’s FFPE RNA/DNA Purification Plus Kit provides a rapid method for the sequential isolation and purification of total RNA (including microRNA) and genomic DNA from formalin-fixed paraffin-embedded (FFPE) tissue samples. RNA and DNA are sequentially purified into separate fractions using this kit. The kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in high quality and yield of RNA/DNA. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as the degree of fragmentation of the RNA will increase over time. RNA is purified without using phenol or chloroform. Purified RNA is of the highest integrity and can be used in a number of downstream applications including real time PCR. Purified genomic DNA is also of the highest quality, and can be used in sequencing.
Research Products International Corp YeaStar Genomic DNA Kit w/Zymo-Spin III Columns, Uncapped, 40 Preps
The YeaStar Genomic DNA Kit is designed for reliable and efficient isolation of genomic DNA from a broad spectrum of fungal species, including Aspergillus fumigatus, Aspergillus nidulans, Aspergillus nivens var. aureus, Candida albicans, Pichia pastoris, Saccharomyces cerevisiae, Schizosaccharomyces pombe, and any fungi whose cell walls are susceptible to yeast lytic enzyme lysis. The kit is based on highly efficient enzyme lysis and Zymo-Spin column technology. Each standard prep yields about 7 - 20 µg of DNA with a size distribution of 35 - 60 kb. The resulting genomic DNA can be used direct analysis including Southern blotting, PCR, restriction endonuclease digestion, etc.
Simple: Fast spin-column procedure yields pure yeast genomic DNA without using glass beads or phenol.
Omega Biotek Inc Endo-Free Plasmid DNA Midi Kit, 25 preps
Endo-Free Plasmid DNA Midi Kit provides an efficient method for the large-scale isolation of low-endotoxin plasmid DNA. The system utilizes Lysate Clearance Filter Syringes instead of centrifugation to rapidly clear bacterial lysates post-alkaline lysis. Following lysate clearance, endotoxins are removed, and the protocol follows a simple “bind-wash-elute” procedure to deliver high-quality plasmid DNA.
Cygnus Technologies CHO Host Cell DNA Detection Kit in Tubes
Used to measure residual CHO host cell DNA, this kit uses a 2 ml microfuge tube format and includes our proprietary extraction procedure, which does not use iodine or glycogen. The superior co-precipitating reagents do not interfere with qPCR and ensure that pellets do not become dislodged during extraction. This eliminates the need to centrifiuge samples or repeat a run. Cell line-specific, CHO DNA calibrators are included in the kit. Upon completion of extraction, samples are reacted with PicoGreen solution. Time to completion is under 2 hours. Each kit supplies enough reagent for the analysis of 50 samples in duplicate.
Abcam DNA Concentrator Kit, 50 Tests
The DNA Concentrator Kit (ab156895) is a complete set of essential components which enables the experimenter to efficiently concentrate DNA from various DNA samples with low concentration of DNA including those from microdissection samples and paraffin-embedded tissues.
The product is subject to the following: Abcam Restricted Use Statement
MDI Membrane FAST ENDOFREE MIDIPREP 25RXN
Endotoxin free Quanta Midiprep plasmid DNA purification kits are certified for low endotoxin levels of < 0.1EU/µg. Thses are specially designed to offer very high yields and purity with much reduced process time. The kit allows fast purification of large quantities of plasmid DNA, from culture pellet, in just 30 minutes. The miniaturized Midi column with high binding capacity special membrane fits into a 1.5 mL collection tube to allow washing and elution of bound plasmid DNA, using a microcentrifuge. The eluate contains highly concentrated plasmid DNA and does not require DNA precipitation and reconstitution, preventing yield losses and salt contamination.
Research Products International Corp Zymoclean Gel DNA Recovery Kit w/ Zymo-Spin IC Columns, Capped, 50 Preps
The Zymoclean Gel DNA Recovery Kit is a gel DNA extraction kit that provides rapid purification of high quality DNA from TAE/TBE-buffered agarose gels. This gel DNA extraction kit features Zymo-Spin technology to yield high-quality, purified DNA in just minutes. DNA purified using the Zymoclean Gel DNA Recovery kits is perfectly suited for use in DNA ligation reactions, sequencing, DNA labeling reactions, PCR, etc.
Quick recovery of ultra-pure DNA from agarose gels.
Column design permits DNA elution at high concentrations into minimal volumes.
Eluted DNA is well-suited for use in DNA ligation, sequencing, labeling, PCR, etc.
Epoch Life Science Inc GenCatch™ Anion Exchange DNA Maxi Columns (50/PK)
Ion exchange columns for transfection grade plasmid DNA purification. Anion-exchange technology. 1000 ug (1 mg) plasmid DNA binding capacity. Gravity flow. Compatible with third party buffer sets.
Epoch Life Science Inc GenCatch™ Plasmid Plus DNA Maxiprep Kit (10 preps) Uniquely formulated buffers, reinforced anion exchange columns with collection tubes, along with protocols.
Provides a simple, fast and cost-effective method to purify transfection grade plasmid DNA using ion (anion) exchange technology without phenol extraction or ethanol precipitation. 500 ug plasmid DNA binding capacity. Gravity flow. DNA prepared by this kit is suitable for many downstream molecular manipulations including PCR, ligation, restriction enzyme digestion, transformation and transfection, radioactive and fluorescent sequencing, etc.
Zymo Research Corporation DNA Binding Buffer, 25ml
This buffer facilities efficient binding and recovery of DNA from enzymatic reactions and impure DNA samples.